Glycosyl compounds
![Research Products International Corp [ONPG] 2-Nitrophenyl-B-D-Galactopyranoside, 5 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-N81000-5.0.JPG-250.jpg)
Research Products International Corp [ONPG] 2-Nitrophenyl-B-D-Galactopyranoside, 5 Grams
Colorimetric and spectrophotometric substrate for detection of β-D-Galactosidase.
eMolecules Medchem Express / Delphinidin (chloride) / 5mg / 624169648 / HY-N2409 / / 528-53-0 / MFCD00016663 / 338.700 / C15H11ClO7
Medchem Express / Delphinidin (chloride) / 5mg / 624169648 / HY-N2409 / / 528-53-0 / MFCD00016663 / 338.700 / C15H11ClO7
![Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 10 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B71800-10.0.JPG-250.jpg)
Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 10 Grams
Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.
Water Content: 0.05% (K. Fisher)
Protect from light and moisture
Gold Biotechnology Inc HEPES, Free Acid 500 g
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay.
Medchemexpress LLC Beta-1 | 2924598-24-1 | C37H28IN3S2 | 1 ML
BETA-1 is the first twisted intramolecular charge transfer (TICT)-aggregation-induced emission (AIE) integration molecule. It emits cyan fluorescence in lipid droplets (LDs) and red fluorescence in mitochondria, enabling simultaneous and dual-color imaging of LDs and mitochondria both in vivo and in vitro.
- Used for imaging of lipid droplets and mitochondria in live cells.
- Enables simultaneous and dual-color imaging of lipid droplets and mitochondria.
- Emits cyan fluorescence in lipid droplets and red fluorescence in mitochondria.
- Applicable in vivo and in vitro studies.
Research Products International Corp Magenta-Gal, 2.5 G
Chromogenic substrate of β-Galactosidase. Produce magenta color.
Chem-Impex International, Inc. 5-Azacytidine | 320-67-2 | MFCD00006539 | 5G
5-Azacytidine, 320-67-2, MFCD00006539, 5G